top of page
メインイメージ5.jpg
研究成果

Publications

論文

  1. Li Y., Wang Q., Jia H., Ishikawa K., Kosami K., Ueba T., Tsujimoto A., Yamanaka M., Yabumoto Y., Miki D., Sasaki E., Fukao Y., Fujiwara M., Kaneko-KAWANO T., Tan L., Kojima C., Wing R., Shimizu M., Terauchi R., Shimamoto K., Sebastian A., Nishimura H., Fukada F., Niu Q., Yoshida K., and Kawano Y. (2024) An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function. Nature Commun., 15(1), 4610.

  2. Takei T, Tsukada M, Tamura K, Hara-Nishimura I, Fukao Y, Kurihara Y, Matsui M, Saze H, Tsuzuki M, Watanabe Y, Hamada T. (2024) ARGONAUTE1-binding Tudor domain proteins function in small interfering RNA production for RNA-directed DNA methylation. Plant Physiol., 336, 111840.

  3. Ushio M., Ishikawa T., Matsuura T., Mori IC, Kawai-Yamada M, Fukao Y. and Nagano M.(2023) MHP1 and MHL generate odd-chain fatty acids from 2-hydroxy fatty acids in sphingolipids and are related to immunity in Arabidopsis thaliana. Plant Sci., 336, 111840.

  4. Kimura S., Vaattovaara A., Ohshita T., Yokoyama K., Yoshida K., Hui A., Kaya H, Ozawa A., Kobayashi M., Mori IC., Ogata Y., Ishino Y., Sugano SS., Nagano M. and Fukao Y. (2023) Zinc deficiency-induced defensin-like proteins are involved in the inhibition of root growth in Arabidopsis. Plant J, 115(4), 1071-1083.

  5. Ishii C., Nagano M., Kobayashi M., Matsuura T., Konishi J., Aoki K., Daimon H., Mori IC. and Fukao Y.(2023) Wood vinegar promotes rice growth by increasing gibberellin and cytokinin levels. The Japan Carbonization Research Society, 1(2), 41-50.

  6. Aryal B, Xia J, Hu Z, Stumpe M, Tsering T, Liu J, Huynh J, Fukao Y, Glöckner N, Huang HY, Sáncho-Andrés G, Pakula K, Ziegler J, Gorzolka K, Zwiewka M, Nodzynski T, Harter K, Sánchez-Rodríguez C, Jasiński M, Rosahl S, Geisler M. (2023) A LRR receptor kinase controls ABC transporter substrate preferences during plant growth-defense decisions. Current Biol., 33(10), 2008-2023.

  7. Ishikawa K., Konno R., Hirano S., Fujii Y., Fujiwara M., Fukao Y. and Kodama Y. (2022) The ER membrane–bending protein RETICULON facilitates chloroplast relocation movement in Marchantia polymorpha. Plant J., 111(1), 205-216.

  8. Ukawa T, Banno F, Ishikawa T, Kasahara K, Nishina Y, Inoue R, Tsujii K, Yamaguchi M, Takahashi T, Fukao Y, Kawai-Yamada M and Nagano M. (2022) Sphingolipids with 2-hydroxy fatty acids aid in plasma membrane nanodomain organization and oxidative burst. Plant Physiol., 189(2), 839-857.

  9. Hasegawa Y, Reyes TH, Uemura T., Baral A., Fujimaki A., Luo Y., Morita Y., Saeki Y., Maekawa S., Yasuda M., Mukuda K.,Fukao Y., Tanaka K., Nakano A., Takagi J., Bhalerao RP., Yamaguchi J. and Sato T. (2022) TGN/EE SNARE protein SYP61 and ubiquitin ligase ATL31 cooperatively regulate carbon/nitrogen-nutrient responses in Arabidopsis. Plant Cell, 34(4), 1354-1374.

  10. Takusagawa M., Kobayashi Y., Fukao Y., Hidaka K., Endo M., Sugiyama H., Hamaji T., Kato Y., Miyakawa I., Misumi O., Shikanai T. and Nishimura Y. (2021) HBD1 protein with a tandem repeat of two HMG box domains is a DNA clip to organize chloroplast nucleoids in Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA, 118(20), e2021053118.

  11. Ayabe H., Kawai N., Shibamura M., Fukao Y., Fujimoto M., Tsutsumi N. and Arimura SI. (2021) FMT, a protein that affects mitochondrial distribution, interacts with translation-related proteins in Arabidopsis thaliana. Plant Cell Reports., 40(2), 327-337.

  12. Yoshinari A, Hosokawa T., Beier M.P., Oshima K., Ogino Y., Hori C., Takasuka T.E., Fukao Y., Fujiwara T. and Takano J. (2021) Transport-coupled ubiquitination of the borate transporter BOR1 for its boron-dependent degradation. Plant Cell, 33(2), 420-438.

  13. Ayabe H., Kawai N., Shibamura M., Fukao Y., Fujimoto M., Tsutsumi N. and Arimura SI. (2021) FMT, a protein that affects mitochondrial distribution, interacts with translation-related proteins in Arabidopsis thaliana. Plant Cell Reports., in press.

  14. Yoshinari A, Hosokawa T., Beier M.P., Oshima K., Ogino Y., Hori C., Takasuka T.E., Fukao Y., Fujiwara T. and Takano J. (2020) Transport-coupled ubiquitination of the borate transporter BOR1 for its boron-dependent degradation. Plant Cell, in press.

  15. Higo A., Saihara N., Miura F., Higashi Y., Yamada M., Tamaki S., Ito T., Tarutani Y., Sakamoto T., Fujiwara M., Kurata T., Fukao Y., Moritoh S., Terada R., Kinoshita T., Ito T., Kakutani T., Shimamoto K. and Tsuji H. (2020) DNA methylation is reconfigured at the onset of reproduction in rice shoot apical meristem. Nature Commun., 11(1), 4079.

  16. Tai C., Sawada Y., Masuda J., Daimon H. and Fukao Y. (2020) Cultivation of Spinach in Hot Seasons using a Micro-mist-based Temperature-control System. Scientia Horticulturae, 273, 109603.

  17. Nakayama S., Sugano S.S., Hirokawa H., Mori I.C., Daimon H., Kimura S. and Fukao Y. (2020) Manganese Treatment Alleviates Zinc Deficiency Symptoms in Arabidopsis Seedlings. Plant Cell Physiol., 61(10), 1711-1723.

  18. Nagano M., Ueda H., Fukao Y., Kawai M. and Hara-Nishimura I. (2020) Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochrome b5-B. Plant Signal Behav., 15(9), e1790196.

  19. Ishikawa K., Tamura K., Fukao Y. and Shimada T. (2020) Structural and functional relationships between plasmodesmata and plant endoplasmic reticulum–plasma membrane contact sites consisting of three synaptotagmins. New Phytol., 226(3), 798-808.

  20. Furutani M., Hirano Y., Nishimura T., Nakamura M., Taniguchi M., Suzuki K., Oshida R., Kondo C., Sun S., Kato K, Fukao Y., Hakoshima T. and Morita M. (2020) Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control. Nature Commun., 11(1), 76.

  21. Wang P., Yao S., Kosami K., Guo T., Li J., Zhang Y., Fukao Y., Kaneko-Kawano T., Zhang H., She Y., Wang P., Xing W., Hanada K., Liu R. and Kawano Y. (2020) Identification of endogenous small peptides involved in rice immunity through transcriptomics- and proteomics-based screening. Plant Biotechnol. J., 18 (2), 415-428.

  22. Luo Y., Aoyama S., Fukao Y., Sato T. and Yamaguchi J. (2019) Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis. Plant Biotech., 36 (2), 107-112.

  23. Goto C, Hashizume S., Fukao Y. and Hara-Nishimura I. and Tamura K. (2019) Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction. Nucleus, 10 (1), 81-92.

  24. Nagano M., Kurata C., Fukao Y. Fujiwara M., Uchimiya H. and Kawai-Yamada M. (2019) Arabidopsis Bax inhibitor-1 interacts with enzymes related to very-long-chain fatty acid synthesis. J. Plant Res., 132 (1), 131-143.

  25. Iwabuchi K.,Ohnishi H.,Tamura K.,Fukao Y.,Furuya T.,Hattori K.,Tsukaya H.and Hara-Nishimura I. (2018) ANGUSTIFOLIA Regulates Actin Filament Alignment for Nuclear Positioning in Arabidopsis Leaves. Plant Physiol., in press

  26. SakamotoT., Tsujimoto-Inui Y., Sotta N., Hirakawa T., Matsunaga T., Fukao Y., Matsunaga S. and Fujiwara T.(2018) Proteasomal degradation of BRAHMA promotes Boron tolerance in Arabidopsis. Nature Commun., in press

  27. Kato Y., Odahara, M., Fukao Y. and Shikanai T.(2018) Stepwise evolution of supercomplex formation with photosystem I is required for stabilization of chloroplast NADH dehydrogenase-like complex: Lhca5-dependent supercomplex formation in Physcomitrella patens. Plant J., 96 (5), 937-948.

  28. Nakaminami K., Okamoto M., Higuchi-Takeuchi M., Yoshizumi T., Yamaguchi Y., Fukao Y., Shimizu M., Ohashi C., Tanaka M., Matsui M., Shinozaki K., Seki M. and Hanada K. (2018) AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants.Proc. Natl. Acad. Sci. USA, 115 (22), 5810-5815.

  29. Noguchi M., Fujiwara M., Sano R., Nakano Y., Fukao Y., Ohtani M. and Demura T. (2018) Proteomic analysis of xylem vessel cell differentiation in VND7-inducible tobacco BY-2 cells by two-dimensional gel electrophoresis.Plant Biotech., 35 (1), 31-37.

  30. Morimoto K., Ohama N., Kidokoro S., Mizoi J., Takahashi F., Todaka D., Mogami J., Sato H., Qin F., Kim J.S., Fukao Y., Fujiwara M, Shinozaki K. and Yamaguchi-Shinozaki K. (2017) BPM-CUL3 E3 ligase modulates thermotolerance by facilitating negative regulatory domain-mediated degradation of DREB2A in Arabidopsis. Proc. Natl. Acad. Sci. USA, in press

  31. Chen P., Takatsuka H., Takahashi N., Kurata R., Fukao Y., Kobayashi K., Ito M. and Umeda M. (2017) Arabidopsis R1R2R3-Myb proteins are essential for inhibiting cell division in response to DNA damage. Nature Commun., 8, 635.

  32. Takagi D., Amako K., Hashiguchi M., Fukaki H., Ishizaki K., Goh T., Fukao Y., Sano R., Kurata T., Demura T., Sawa S., Miyake C. (2017) Chloroplastic ATP synthase builds up proton motive force for preventing reactive oxygen species production in photosystem I. Plant J., 91 (2), 306-324.

  33. Tamura K., Fukao Y., Hatsugai N., Katagiri F. and Hara-Nishimura I. (2017) Nup82 functions redundantly with Nup136 in a salicylic acid-dependent defense response of Arabidopsis thaliana. Nucleus, 8 (3), 301-311.

  34. Yamada K., Yamaguchi K., Shirakawa T., Nakagami H., Mine A., Ishikawa K., Fujiwara M., Narusaka M., Narusaka Y., Ichimura K., Kobayashi Y., Matsui H., Nomura Y., Nomoto M., Tada Y., Fukao Y., Fukumizo T., Tsuda K., Shirasu K, Shibuya N and Kawasaki T. (2016) The CERK1-associated kinase PBL27 mediates 1 chitin-triggered MAPK activation in Arabidopsis. EMBO J., 35 (22), 2468-2483.

  35. Yokoyama R., Yamamoto H., Kondo M., Takeda S., Ifuku K., Fukao Y., Kamei Y., Nishimura M. and Shikanai T. (2016) Grana-localized Proteins, RIQ1 and RIQ2, Affect the Dynamics of Light-harvesting Complex II and Grana Stacking in Arabidopsis. Plant Cell, 28 (9), 2261-2275.

  36. Nagano M., Ishikawa T., Fujiwara M., Fukao Y., Kawano Y., Kawai-Yamada M. and Shimamoto K. (2016) Plasma membrane microdomains are essential for OsRac1-OsRbohB/H-mediated immunity in rice. Plant Cell, 28 (8), 1966-1983.

  37. Yamamoto H., Fan X., Sugimoto K., Fukao Y., Peng L. and Shikanai T. (2016) CHLORORESPIRATORY REDUCTION 9 Is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-like Complex. Plant Cell Physiol., 57 (10）, 2122-2132.

  38. Lu Y., Yasuda S., Li X., Fukao Y., Tohge T., Fernie A.R., Matsukura C., Ezura H., Sato T. and Yamaguchi J. (2016) Characterization of ubiquitin ligase SlATL31 and proteomic analysis of 14-3-3 targets in tomato fruit tissue (Solanum lycopersicum L.). J. Proteomics, 143, 254-264.

  39. Zhu J., Bailly A., Zwiewka M., Sovero V., Di Donato M.,Ge P., Oehri J., Aryal B., Hao P., Linnert M., Inés Burgardt N., Lücke C., Weiwad M., Michel M., Weiergräber O.H., Pollmann S., Azzarello E., Mancuso S., Ferro N., Fukao Y., Hoffmann C., Wedlich-Söldner R., Friml J., Thomas C. and Geisler M. (2016) TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics. Plant Cell, 28 (4), 930-948.

  40. Reuscher S.†, Fukao Y. †, Morimoto R., Otagaki S., Oikawa A., Isuzugawa K. and Shiratake K.(2016）Quantitative proteomics based reconstruction and identification of metabolic pathways and membrane transport proteins related to sugar accumulation in developing fruits of Pear (Pyrus communis）. Plant Cell Physiol., 57 (3）, 505-518.† these authors contributed equally to this work.

  41. Fukao Y.*, Kobayashi M., Zargar SM., Kurata R., Fukui R. Mori CI. and Ogata Y. (2016）Quantitative proteomic analysis of the response to zinc, magnesium, and calcium deficiency in specific cell types of Arabidopsis roots.Proteomes, 4 (1）, 1-13.

  42. Ueda H., Yokota E., Kuwata K., Kutsuna N., Mano S., Shimada T., Tamura K., Stefano G., Fukao Y., Brandizzi F., Shimmen T., Nishimura M. and Hara-Nishimura I. (2016）Phosphorylation of the c-terminus of RHD3 has a critical role in homotypic ER membrane fusion in Arabidopsis.Plant Physiol., 170 (2）, 867-880.

  43. Kobayashi Y., Takusagawa M., Harada N., Fukao Y., Yamaoka S., Kohchi T., Hori K., Ohta H., Shikanai T. and Nishimura Y. (2015）Eukaryotic components remodeled chloroplast nucleoid organization during the green plant evolution. Genome Biol Evol., 8 (1）, 1-16.

  44. Fuji K., Shirakawa M., Shimono Y., Kunieda T., Fukao Y., Koumoto Y., Takahashi H., Hara-Nishimura I. and Shimada T. (2015）The adaptor complex AP-4 regulates vacuolar protein sorting at trans-Golgi network by interacting with VACUOLAR SORTING RECEPTOR 1.Plant Physiol., 170 (1）, 211-219.

  45. Suzuki M., Takahashi S., Kondo T., Dohra H., Ito Y., Kiriiwa Y., Hayashi M., Kamiya S., Kato M., Fujiwara M., Fukao Y., Kobayashi M., Nagata N. and Motohashi R. (2015）Plastid proteomic analysis in tomato fruit development. PLoS One, 10 (9）, e0137266.

  46. Inaba S., Kurata R., Kobayashi M., Yamagishi Y., Mori I., Ogata Y. and Fukao Y.* (2015）Identification of putative target genes of bZIP19, a transcription factor essential for Arabidopsis adaptation to Zn deficiency in roots. Plant J., 84 (2）, 323-334.

  47. Tsujimura K., Irie K., Nakashima H., Egashira Y., Fukao Y., Fujiwara M., Itoh M., Uesaka M., Imamura T., Nakahata Y., Yamashita Y., Abe T., Takamori S. and Nakashima K. (2015）miR-199a links MeCP2 with mTOR signaling and its dysregulation causes Rett syndrome phenotypes. Cell Rep., 12 (11）, 1887-1901.

  48. Moro C.F., Fukao Y., Shibato J., Rakwal R., Agrawal GK., Shioda S., Kouzuma Y. and Yonekura M. (2015）Immature seed endosperm and embryo proteomics of the lotus (Nelumbo nucifera Gaertn.）by one-dimensional gel-based tandem mass spectrometry and a comparison with the mature endosperm proteome. Proteomes, 3 (3）, 184-235.

  49. Shi Q., Araie H., Bakku RK., Fukao Y., Rakwal R., Suzuki I. and Shiraiwa Y. (2015）Proteomic analysis of lipid body from the alkenone-producing marine haptophyte alga Tisochrysis lutea. Proteomics., 15 (23-24）, 4145-4158.

  50. Fukao Y.* (2015）Discordance between protein and transcript levels detected by selected reaction monitoring. Plant Signal Behav., 10 (5）, e1017697.

  51. Zargar SM., Kurata R., Inaba S., Oikawa A., Fukui R., Ogata Y., Agrawal GK., Rakwal R. and Fukao Y.* (2015）Quantitative proteomics of Arabidopsis shoot microsomal proteins reveals a cross-talk between excess zinc and iron deficiency. Proteomics., 15 (7）, 1196-1201.

  52. Moro C.F., Fukao Y., Shibato J., Rakwal R., Timperio A.M., Zolla L., Agrawal GK., Shioda S., Kouzuma Y. and Yonekura M. (2015）Unraveling the seed endosperm proteome of the lotus (Nelumbo nucifera Gaernt.）utilizing 1DE and 2DE separation in conjunction with tandem mass spectrometry. Proteomics., 15 (10）, 1717-1735.

  53. Huang J., Fujimoto M., Fujiwara M., Fukao Y., Arimura S. and Tsutsumi N. (2015）Arabidopsis dynamin-related proteins, DRP2A and DRP2B, function coordinately in post-Golgi trafficking. Biochem. Biophys. Res. Commun., 456 (1）, 238-244.

  54. Zargar SM., Fujiwara M., Inaba S., Kobayashi M., Kurata R., Ogata Y. and Fukao Y.* (2015）Correlation analysis of proteins responsive to Zn, Mn, or Fe deficiency in Arabidopsis roots based on iTRAQ analysis. Plant Cell Rep., 34 (1）, 157-166.

  55. Ido K., Nield J., Fukao Y., Nishimura T., Sato F. and Ifuku K. (2014）Cross-linking evidence for multiple interactions of the PsbP and PsbQ proteins in a higher plant photosystem II supercomplex. J. Biol. Chem., 289 (29）, 20150-20157.

  56. Sako K., Yanagawa Y., Kanai T., Sato T., Seki M., Fujiwara M., Fukao Y. and Yamaguchi J. (2014）Proteomic analysis of 26S proteasome reveals direct interaction with transit peptides of plastid protein precursors for degradation. J. Proteome Res., 13 (7）, 3223-3230.

  57. Goto C., Tamura K., Fukao Y., Shimada T. and Hara-Nishimura I. (2014）The novel nuclear envelope protein KAKU4 modulates nuclear morphology in Arabidopsis thaliana. Plant Cell, 26 (5）, 2143-2155.

  58. Yasuda S., Sato T., Maekawa S., Aoyama S., Fukao Y. and Yamaguchi J. (2014）Phosphorylation of Arabidopsis ubiquitin ligase ATL31 is critical for plant C/N-nutrient response and controls the stability of 14-3-3 proteins. J. Biol. Chem., 289 (22）, 15179-15193.

  59. Entani T., Kubo K., Isogai S., Fukao Y., Shirakawa M., Isogai A. and Takayama S. (2014）Ubiquitin-proteasome–mediated degradation of S-RNase in a solanaceous cross-compatibility reaction, Plant J., 78 (6）, 1014-1021.

  60. Fujiwara M., Uemura T., Ebine K., Nishimori Y., Ueda T., Nakano A., Sato MH. and Fukao Y.* (2014）Interactomics of Qa-SNARE in Arabidopsis thaliana. Plant Cell Physiol., 55 (4）, 781-789.

  61. Tanaka N., Kato M., Tomioka R., Kurata R., Fukao Y., Aoyama T. and Maeshima M. (2014）Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses. J. Exp. Bot., 65 (6）, 1497-1512.

  62. Maekawa S., Inada N., Yasuda S., Fukao Y., Fujiwara M., Sato T. and Yamaguchi J. (2014）The C/N regulator ATL31 controls papilla formation in response to powdery mildew fungi penetration by interacting with SNARE SYP121 in Arabidopsis. Plant Physiol., 164 (2）, 879-887.

  63. Shimada TL., Takano Y., Shimada T., Fujiwara M., Fukao Y., Mori M., Okazaki Y., Saito K., Sasaki R., Aoki K. and Hara-Nishimura I. (2014）Leaf oil body functions as a subcellular factory for the production of a phytoalexin in Arabidopsis thaliana. Plant Physiol., 164 (1）, 105-118.

  64. Hamada T., Nagasaki-Tekeuchi N., Kato T., Fujiwara M., Sonobe S., Fukao Y. and Hashimoto T. (2013）Purification and characterization of novel microtubule-associated proteins from Arabidopsis cell suspension cultures. Plant Physiol., 163 (4）, 1804-1816.

  65. Tamaki T., Betsuyaku S., Fujiwara M., Fukao Y., Fukuda H. and Sawa S. (2013）SUPPRESSOR OF LLP1 1-mediated C-terminal processing is critical for CLE19 peptide activity. Plant J., 76 (6）, 970-981.

  66. Takagi J., Renna L., Takahashi H., Koumoto Y., Tamura K., Stefano G., Fukao Y., Kondo M., Nishimura M., Shimada T., Brandizzi F. and Hara-Nishimura I. (2013）MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell, 25 (11）, 4658-4675.

  67. Sun H.H., Fukao Y., Ishida S., Yamamoto H., Maekawa S., Fujiwara M., Sato T. and Yamaguchi J. (2013）Proteomics analysis reveals a highly heterogenous proteasome composition and the post-translational regulation of peptidase activity under pathogen signaling in plants. J. Proteome Res., 12 (11）, 5084-5095.

  68. Zargar SM., Kurata R., Inaba S. and Fukao Y.* (2013）Unraveling the iron deficiency responsive proteome in Arabidopsis shoot by iTRAQ-OFFGEL approach. Plant Signal Behav., 9 (1）, e26892.

  69. Yoshida K., Ohnishi M., Fukao Y., Okazaki Y., Fujiwara M., Song C., Nakanishi Y., Saito K., Shimmen T., Suzaki T., Hayashi F., Fukaki H., Maeshima M. and Mimura T. (2013）Studies on vacuolar membrane microdomains isolated from Arabidopsis suspension-cultured cells: Local distribution of vacuolar membrane proteins. Plant Cell Physiol., 54 (10）, 1571-1584.

  70. Tamura K., Iwabuchi K., Fukao Y., Kondo M., Okamoto K., Ueda H., Nishimura M. and Hara-Nishimura I. (2013）Myosin XI-i links the nuclear membrane with the cytoskeleton for nuclear movement and shape in Arabidopsis thaliana. Curr. Biol., 23 (18）, 1776-1781.

  71. Yamaoka S., Shimono Y., Shirakawa M., Fukao Y., Kawase T., Hatsugai N., Tamura K., Shimada T. and Hara-Nishimura I. (2013）Identification and dynamics of Arabidopsis AP-2 complex and its involvement in floral organ development. Plant Cell, 25 (8）, 2958-2969.

  72. Teh OK., Shimono Y., Shirakawa M., Fukao Y., Tamura K., Shimada T. and Hara-Nishimura I. (2013）The AP-1 μ adaptin is required for KNOLLE localization at the cell plate to mediate cytokinesis in Arabidopsis. Plant Cell Physiol., 54 (6）, 838-847.

  73. Hoshiyasu S., Kohzuma K., Yoshida K., Fujiwara M., Fukao Y., Yokota A. and Akashi K. (2013）Potential involvement of N-terminal acetylation in the quantitative regulation of the ε subunit of chloroplast ATP synthase under drought stress. Biosci. Biotechnol. Biochem., 77 (5）, 998-1007.

  74. Fukao Y.*, Yoshida M., Kurata R., Kobayashi M., Nakanishi M., Fujiwara M., Nakajima K. and Ferjani A. (2013）Peptide separation methodologies for in-depth proteomics in Arabidopsis. Plant Cell Physiol., 54 (5）, 808-815.

  75. Cui S, Fukao Y., Mano S, Yamada K., Hayashi M. and Nishimura M. (2013）Proteomic Analysis Reveals that the Rab GTPase RabE1c is involved in the degradation of the peroxisomal protein receptor PEROXIN 7. J. Biol. Chem., 288 (8）, 6014-6023.

  76. Nakano R.T., Matsushima R., Nagano A.J., Fukao Y., Fujiwara M., Kondo M., Nishimura M. and Hara-Nishimura I. (2012）ERMO3/MVP1/GOLD36 is involved in a cell type-specific mechanism for maintaining ER morphology in Arabidopsis thaliana. PLoS One, 7 (11）, e49103.

  77. Nakasone A., Fujiwara M., Fukao Y., Biswas, KK., Kawai-Yamada M., Narumi I., Uchimiya H. and Oono Y. (2012）SMALL ACIDIC PROTEIN 1 acts with RUB modification components, the COP9 signalosome and AXR1, to regulate growth and development of Arabidopsis thaliana. Plant Physiol., 160 (1）, 93-105.

  78. Ido K., Kakiuchi S., Uno C., Nishimura T., Fukao Y., Noguchi T., Sato F. and Ifuku K. (2012）The conserved His144 in the PsbP protein is important for the interaction between the PsbP N-terminus and the Cyt B559 subunit of photosystem II. J. Biol. Chem., 287 (31）, 26377-26387.

  79. Henrichs S., Wang B., Fukao Y., Zhu J., Charrier L., Bailly A., Oehring SC., Linnert M., Weiwad M., Endler A., Nanni P., Pollmann S., Mancuso S., Schulz A. and Geisler M. (2012）Regulation of ABCB1/PGP1-catalysed auxin transport by linker phosphorylation. EMBO J., 31 (13）, 2965-2980.

  80. Mano S, Nakamori C., Fukao Y., Araki M., Matsuda A., Kondo M. and Nishimura M. (2011）The defect of peroxisomal membrane protein 38 causes enlargement of peroxisomes. Plant Cell Physiol., 52 (12）, 2157-2172.

  81. Sato T., Maekawa S., Yasuda S., Domeki Y., Sueyoshi K., Fujiwara M., Fukao Y., Goto DB. and Yamaguchi J. (2011）Identification of 14-3-3 proteins as a target of ATL31 ubiquitin ligase, a regulator of the C/N response in Arabidopsis. Plant J., 68 (1）, 137-146.

  82. Fukao Y.* and Ferjani A. (2011）V-ATPase dysfunction under excess zinc inhibits Arabidopsis cell expansion. Plant Signal. Behav., 6 (9）, 1253-1255.

  83. Fukao, Y.*, Ferjani A., Tomioka R., Nagasaki N., Kurata R., Nishimori Y., Fujiwara M. and Maeshima M. (2011）iTRAQ analysis reveals mechanisms of growth defects due to excess zinc in Arabidopsis. Plant Physiol., 155 (4）, 1893-1907.

  84. Yamamoto H. Peng L., Fukao Y. and Shikanai T. (2011）An srchomology 3 domain-like fold protein forms a ferredoxin-binding site for the chloroplast NADH dehydrogenase-like complex in Arabidopsis. Plant Cell, 23 (4）, 1480-1493.

  85. Tamura K., Fukao Y., Iwamoto M., Haraguchi T. and Ikuko Hara-Nishimura I. (2010）Identification of nuclear pore complex components in Arabidopsis thaliana. Plant Cell, 22 (12）, 4084-4097.

  86. Igawa T., Fujiwara M., Tanaka I., Fukao, Y. and Yanagawa Y. (2010）Characterization of bacterial-type phosphoenolpyruvate carboxylase expressed in male gametophyte of higher plants. BMC Plant Biol., 14 (10）200.

  87. Okuda K., Hammani K., Tanz S., Peng L., Fukao Y., Myouga F., Motohashi R., Shinozaki K., Small I. and Shikanai T. (2010）The pentatricopeptide repeat protein OTP82 is required for RNA editing of plastid ndhB and ndhG transcripts. Plant J., 61 (2）, 339-349.

  88. Fukao Y.*, Ferjani A., Fujiwara M., Nishimori Y. and Ohtsu I. (2009）Identification of zinc-responsive proteins in the roots of Arabidopsis thaliana using a highly improved method of two-dimensional electrophoresis., Plant Cell Physiol., 50 (12）, 2234-2239.

  89. Sato A., Sato Y., Fukao Y., Fujiwara M., Umezawa T., Shinozaki K., Hibi T., Taniguchi M., Miyake H., Goto DB. and Uozumi N. (2009）Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase., Biochem J., 424 (3）, 439-448.

  90. Peng L., Fukao Y., Fujiwara M., Takami T. and Shikanai T. (2009）Efficient operation of NAD(P）H dehydrogenase-dependent cyclic electron flow requires the supercomplex formation with photosystem I via minor LHCI in Arabidopsis. Plant Cell, 21 (11）, 3623-3640.

  91. Fujiwara M., Hamada S., Hiratsuka M., Fukao Y., Kawasaki T. and Shimamoto K. (2009）Proteome analysis of detergent-resistant membranes (DRMs）associated with OsRac1-mediated innate immunity in rice. Plant Cell Physiol., 50 (7）, 1191-1200.

  92. Igawa T., Fujiwara M., Takahashi H., Sawasaki T., Endo Y., Seki M., Shinozaki K., Fukao Y. and Yanagawa Y. (2009）Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings. J. Exp. Bot., 60 (11）, 3067-3073.

  93. Hamada T., Igarashi H., Taguchi R., Fujiwara M., Fukao Y., Shimmen T., Yokota E. and Sonobe S. (2009）The putative RNA-processing protein, THO2, is a microtubule-associated protein in Tobacco. Plant Cell Physiol., 50 (4）, 801-811.

  94. Minami A., Fujiwara M., Furuto A., Fukao Y., Yamashita,T., Kamo M., Kawamura Y. and Uemura M. (2009）Alterations in detergent-resistant plasma membrane microdomains in Arabidopsis thaliana during cold acclimation. Plant Cell Physiol., 50 (2）, 341-359.

  95. Shimada TL., Shimada T., Takahashi H., Fukao Y. and Hara-Nishimura I. (2008）A novel role for oleosins in freezing tolerance of oilseeds in Arabidopsis thaliana. Plant J., 55 (5）, 798-809.

  96. Nagano AJ., Fukao Y., Fujiwara M., Nishimura M. and Hara-Nishimura I. (2008）Antagonistic jacalin-related lectins regulate the size of ER body-type β-glucosidase complexes in Arabidopsis thaliana. Plant Cell Physiol., 49 (6）, 969-980.

  97. Yamada K., Fukao Y., Hayashi M., Fukazawa M., Suzuki I. and Nishimura M. (2007）Cytosolic HSP90 regulates the heat shock response that is responsible for heat acclimation in Arabidopsis thaliana. J. Biol. Chem., 282 (52）, 37794-37804.

  98. Li L., Shimada T., Takahashi H., Ueda H., Fukao Y., Kondo M., Nishimura M. and Hara-Nishimura I. (2006）MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana. Plant Cell, 18 (12）, 3535-3547.

  99. Santelia D., Vincenzetti V., Azzarello E., Bovet L., Fukao Y., Duchtig P., Mancuso S., Martinoia E. and Geisler M. (2005）MDR-like ABC transporter AtPGP4 is involved in auxin-mediated lateral root and root hair development. FEBS Letter, 579 (24）, 5399-5406.

  100. Matsushima R., Fukao Y., Nishimura M. and Hara-Nishimura I. (2004）NAI1 gene encodes a basic-helix-loop-helix-type putative transcription factor that regulates the formation of an endoplasmic reticulum-derived structure, the ER body. Plant Cell, 16 (6）, 1536-1549.

  101. Fukao Y., Hayashi M., Hara-Nishimura I. and Nishimura M. (2003）Novel glyoxysomal protein kinase, GPK1, identified by proteomic analysis of glyoxysomes in etiolated cotyledons of Arabidopsis thaliana (Rapid paper）. Plant Cell Physiol., 44 (10）, 1002-1012.

  102. Fukao Y., Hayashi M. and Nishimura M. (2002）Proteomic analysis of leaf peroxisomal proteins in greening cotyledons of Arabidopsis thaliana (Rapid paper）. Plant Cell Physiol., 43 (7）, 689-696.

  103. Fukao Y., Hayashi Y., Mano S., Hayashi M. and Nishimura M. (2001）Developmental analysis of a putative ATP/ADP carrier protein localized on glyoxysomal membranes during the peroxisome transition in pumpkin cotyledons. Plant Cell Physiol., 42 (8）, 835-841.

書籍

  1. Zargar S.M., Kurata R., Rakwal R. and Fukao Y.* (2015）Peptide separation methodologies for in-depth proteomics. Plant Cell Growth and Expansion - Methods and Protocols. Book Chapter, Edited by Jose M. Estevez, Springer., 1242, pp. 195-209. Springer.

  2. Zargar S.M., Bhattacharjee C., Rai R., Fukao Y., Agarwal G.K. and Rakwal R. (2014）Omics based approaches for improvement of common bean - Omics technologies and crops improvement - Book Chapter, Edited by Noureddine Benkeblia, pp. 271-302, CRC press

  3. Arai Y., Fukao Y., Hayashi M. and Nishimura M. (2008）Plant proteomics: technologies, strategies, and applications. Edited by Agrawal, G.K. and Rakwal, R. pp. 377-389. John Wiley & Sons, Inc., Hoboken, New Jersey.

  4. 深尾　陽一朗、西村　幹夫 (2003）プロテオーム解析から見たペルオキシソーム代謝（総説）、蛋白質核酸酵素（増刊号）、45 (15 Suppl）, 共立出版、pp. 2176-2183.　 Fukao Y. and Nishimura M. (2003）The metabolic survey of plant peroxisomes from proteomic analysis. Tanpakushitsu Kakusan Koso, 48 (15 Suppl）, 2176-2183.

総説

  1. Fukao Y.* (2012）Protein-protein interactions in plants. Plant Cell Physiol., 53 (4）, 617-625.

その他

  1. Zargar SM., Agrawal GK., Rakwal R. and Fukao Y. (2015）Quantitative proteomics reveals role of sugar in decreasing photosynthetic activity due to Fe deficiency. Front. Plant Sci., 6, 592.

  2. Agrawal G.K., Job D., Kieselbach T., Barkla B.K., Chen S., Deswal R., Luthje S., Amalraj R.S., Tanou G., Ndimba B.K., Cramer R., Weckwerth W., Wienkoop S., Dunn M.J., Kim S.T., Fukao Y., Yonekura M., Zolla L., Rohila J.S., Waditee-Sirisattha R., Masi A., Wang T., Sarkar A., Agrawal R., Renaut J. and Rakwal R. (2013）INPPO Actions and Recognition as a Driving Force for Progress in Plant Proteomics: Change of Guard, INPPO Update, and Upcoming Activities. Proteomics, 13 (21）, 3093-3100.

研究費

深尾

​

外部資金

​

・G-7奨学財団（2024年度）

　研究課題名：異科接ぎ木を促進するペプチドの探索

 

・科研費　挑戦的研究（萌芽）（2022〜2023年度）

　研究課題名：タバコ属植物において異科接ぎ木成立を促進する候補ペプチドの作用機作解明

​​​

・山田科学振興財団（2022年度）

　研究課題名：亜鉛欠乏したシロイヌナズナの根端細胞で蓄積する未知構造体の生理的意義の解明

​

・滋賀県近未来技術等社会実装推進事業補助金（2022年度）

　研究課題名：地域および教育機関との連携による家庭生ゴミのコンポスト化によるCO2排出量削減と資源循環モデルの実証（分担）

​

・農林水産省研究推進事業委託プロジェクト研究（2020年〜2024年度）

　研究課題名：農地土壌の炭素貯留能力を向上させるバイオ炭資材の開発（分担）​

​

・科研費　基盤（C）（2019〜2021年度）

　研究課題名：植物の亜鉛恒常性維持に寄与するペプチドと受容体の機能解明

​

・イノベーション創出強化研究推進事業　応用研究ステージ（2019〜2021年度）

　研究課題名：画期的機能を持つ接ぎ木システムの実用化と接ぎ木効率を向上させる接ぎ木促進剤の開発（分担）

​

・平成28年度　農林水産業・食品産業科学技術研究推進事業　シーズ創出ステージ（2016～2018年度）

    研究課題名：画期的機能を持つ野菜の接ぎ木システムの実用化と接ぎ木効率を向上させる接ぎ木接着剤の開発（分担）

​

・第27回加藤記念研究助成（平成28年度～29年度）

    研究課題名：ペプチドを利用した作物の亜鉛欠乏改善

​

・基盤研究（C）（平成26年度～28年度）

    研究課題名：植物の根において亜鉛欠乏を感知するセンサータンパク質の探索とその機能解析

​

・萌芽研究（平成24年度～25年度）

    研究課題名：プロテインホスファターゼが標的とするタンパク質の網羅的解析

​

・新学術領域：環境突破力（馬領域代表:平成23年度～24年度）

    研究課題名：ミネラル欠乏ストレス時に根で機能する輸送体の包括的解析（公募班）

​

・特定領域：オルガネラ分化（西村領域代表：平成19年度～20年度）

    研究課題名：植物における亜鉛に応答した細胞内膜輸送系のプロテオーム解析（公募班）

​

学内資金

​

・国際共同研究促進プログラム（2024年度）

　研究課題名：亜鉛欠乏したシロイヌナズナの根に蓄積するデンプンの生理的意義

​

・グラスルーツ・イノベーションプログラム（GRIP）（2023年度）

　研究課題名：作物栽培において窒素肥料低減を実現する農法の確立

​

・立命館グローバル･イノベーション研究機構　拠点形成型R-GIRO研究プログラム（2021～2025年度）

　研究課題名：気候変動に対応する生命圏科学の基盤創生（分担）

​

​・立命館グローバル･イノベーション研究機構　拠点形成型R-GIRO研究プログラム（2016～2020年度）

　研究課題名：90億人時代に向けた気候変動対応型農業の基盤創生（分担）​

​

・2017年度研究成果国際発信プログラム（平成29年度 後期）

　研究課題名：亜鉛欠乏した植物で機能するペプチドの機能解析

​

​

長野

​

・科研費　基盤（C）（令和2年度～令和4年度）

　研究課題名：細胞膜ナノドメインを基軸とする植物免疫経路の解明（長野、代表）

​

​・科研費　挑戦的研究（萌芽）（平成30年度～令和2年度）

　研究課題名：植物免疫解明に向けたマイクロドメイン可視化プローブの開発（長野、分担）

​

​・研究成果国際発信プログラム　（令和2年度）

　研究課題名：新規植物ナノドメイン可視化プローブの開発とその利用によるナノドメイン動態と制御機構の解明（長野、代表）​

研究費

・平成26年度日本植物学会 特別賞（教育）
　奈良先端大・植物ユニットおよび植物グローバルにより実施された教育プロジェクトとして受賞

招待講演

・国際学会

  1. Fukao Y. “Quantitative proteomics of responsive membrane proteins to nutrient deficiency” International Workshop on Plant Membrane Biology 2013

  2. Fukao Y. “Proteomics in Plant Science -From model to non-model plants-” 19th International Mass Spectrometry Conference, Mascot Workshop 2012

 

・国内学会

  1. 深尾陽一朗「シロイヌナズナにおいて亜鉛欠乏応答するDefensin-like family proteinsの解析」植物の栄養研究会 第6回交流会

  2. 深尾陽一朗，「亜鉛欠乏した植物において機能するペプチド群の探索」， 植物の栄養研究会 第4回研究交流会

  3. 深尾陽一朗「ミネラル欠乏に応答するタンパク質の定量プロテオミクス」、日本プロテオーム学会2011年度大会シンポジウム

  4. 深尾陽一朗「プロテオミクスから見た過剰亜鉛応答」、土壌肥料学会2010年度大会サテライトシンポジウム

  5. 深尾陽一朗「LTQ-Orbitrap XLを用いた核膜孔複合体のハイスループット解析」、第61回日本細胞生物学会　Thermo Fisher SCIENTIFIC主催ランチョンセミナー

 

・研究会

  1. 深尾陽一朗「非モデル植物におけるプロテオーム解析」Second Fascination of Plant Day (FoPD）event

  2. 深尾陽一朗「定量プロテオミクスとバイオインフォマティクスの統合を目指して」、平成24年度生研センターGLPプロジェクト推進会議

  3. 深尾陽一朗「プロテオミクスの成否はデータベースにあり～核プロテオミクスを例に～」、生研センターGLPプロジェクト情報交換会

bottom of page